Abstract

Aim: The aim of this study was to detect three tetracycline resistant genes; tet(K), involved in the production of efflux proteins, as well as tet(M) and tet(O), involved in the production of ribosomal protective proteins, using PCR in previously isolated environmental Gram-positive bacterial strains described as nosocomial from veterinary clinical units.

Method and Materials: Thirty five Gram-positive coccid bacterial strains were used, obtained in a previous study described as nosocomial and phenotypically resistant (R) or with intermediate sensitivity (SI) to tetracyclines determined by Kirby Bauer diffusion antibiogram, in front of a panel of 11 antimicrobials.

Results: Gene were detected as tet (K) gene twice; in 2/22, tet gene (M) in seventeen opportunities; in 10/22 and tet (O) gene twelve times; in 7/22 of the strains resistant to tetracycline and doxycycline.             The tet (K), tet (M) and tet (O) genes were detected simultaneously at one time; in 1/22, tet (K) and tet (O) genes were detected simultaneously at one time; in 1/22 and tet (M) and tet (O) genes were detected simultaneously on six occasions; in 4/22 of the strains resistant to tetracycline and doxycycline; in 1/4 and 1/7 of the strains with intermediate sensitivity to tetracycline and doxycycline respectively.

Conclusion: It was confirmed that methodology allowed to detect three genes most associated with tetracycline resistance in bacteria described as nosocomial.

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