Abstract

Aim: The study was aimed to predict the effects of single nucleotide polymorphism in canine cytochrome b5 reductase using computational methods. 

Method and Materials: Data was obtained from database of National Centre for Biotechnology Information (db SNP) and computational software were used for the analysis.

Results: The 3D protein structure was predicted using  phyre 2 server.  PANTHER analysis predicted the effect of single nucleotide polymorphism (substitution of Isoleucine for Leucine  at position 194 ) as damaging. Analysis using the Mutpred 2 web application also indicated deleterious effects of the amino acid substitution. Molecular mechanisms of structural changes in the amino acid were determined using Mutpred 2 to be altered ordered interface, gain of allosteric sites and altered metal binding.

Conclusion: The study indicated that the substitution of Isoleucine by Leucine at position 194 of the amino acid sequence (Ile 194 Leu) resulted in the destabilization of the amino acid structure leading to functional deviation in canine cytochrome b5 reductase.

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