Archive

Original research (Published On: 27-Nov-2023)

Effects of ß-glucan and vitamin C on immunological and antioxidant serum parameters in rats administered with oral capecitabine

Aysen Altiner, Duygu Kavadarli-Gun, Gokhan Gun and Tanay Bilal

J. Vet. Res. Adv., 05 (02):61-67

Aysen Altiner: Department of Biochemistry, Faculty of Veterinary Medicine, Istanbul University-Cerrahpaşa

Duygu Kavadarli-Gun: Department of Biochemistry, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa

Gokhan Gun: Department of Molecular Biology and Genetics, Institute of Graduate Studies in Science and Engineering, Bogazici University

Tanay Bilal: Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa

Download PDF Here

Article History: Received on: 07-Aug-23, Accepted on: 20-Nov-23, Published on: 27-Nov-23

Corresponding Author: Aysen Altiner

Email: aysen.altiner@iuc.edu.tr

Citation: Kavadarli-Gun D, Altiner A, Gun G and Bilal T (2023). Effects of ß-glucan and vitamin C on immunological and antioxidant serum parameters in rats administered with oral capecitabine. J. Vet. Res. Adv., 05 (02):61-67


Abstract

Aim: Based main purpose of the study to evaluate the effect of β-glucan and vitamin C on antioxidant serum enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), immunoglobulin, an antibody and the important cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in rats administered capecitabine.

Method and materials:  In this study, 35 Sprague Dawley rats were divided into 5 groups of seven. Tween 20 (5 ml/kg body weight/day) to the first group (Group I), 359 mg/kg body weight/day capecitabine to the second group (Group II),  100 mg/kg body weight/day β-glucan in addition to capecitabine to the third group (Group III), 100 mg/kg body weight/day vitamin C in addition to capecitabine to the fourth group (Group IV), and  β-glucan and vitamin C in addition to capecitabine to the fifth group (Group V) were orally given by gastric tube for 14 days. Sera obtained from blood taken from rats on the 14th and 21st days of the experiment were stored at -80 ˚C.

Results: Serum IgG concentrations were significantly higher in Group II given capecitabine on day 14 of blood collection than in other groups. On day 21, IL-1β levels in Groups IV and V were significantly higher than Groups I and II, while IL-1β levels between Groups I, II and III were not significantly different. On the 14th blood draw day, SOD activities in Groups III, IV and V were found to be significantly lower than Groups I and II. On the 14th day of the study, serum CAT activities were found to be significantly lower in Group II given capecitabine than Group IV and V, while the levels in Group IV were found to be significantly higher than the control group (Group I). Again, on the 14th day of the study, a statistically significant difference in serum GPx activities was detected between the control group (44.3 ± 2.64 ng/ml) and Group III (28.7 ± 11.34 ng/ml). On the 14th and 21st days of the study, TNF-α concentrations were found to be significantly lower in the control group (Group I) than in the other groups.

Conclusion: It was concluded that the immunosuppressive feature of chemotherapy in this study caused certain decreases in the levels of antioxidant enzymes, as expected. It seems that chemotherapy suppressed the production of all antioxidant enzymes, but this was different for some cytokines and antibodies. It was observed that therapy with β-glucan, vitamin C and a combination of both showed different effects for each parameter or did not have a specific effect.


Statistics

Article View: 320
PDF Downloaded: 5