Abstract

Aim: The study was to determine the dynamics of some pro-inflammatory cytokines in cocks experimentally infected with Pasteurella multocida.

Method and materials: A total of 40, eighteen-week-old ISA Brown commercial cocks were used in this study. The birds were randomly assigned to two groups (infected and control) of 20 cocks each. Each bird of the infected group was challenged with dose of 0.5 mL of the inoculum containing 12x10⁸ CFU/mL of Pasteurella multocida intra-muscularly using insulin syringes. Blood (3ml) was collected from each of the birds in the infected and control groups via the brachial vein, using 5-mL syringes and 23 G needles, on day 0 (pre-infection), and subsequently, on days 4, 7, 14, 21, 28, 35 and 42 post-infection (pi). Exactly 1 mL of the blood was used for hematological analyses, while the other 2 mL of the blood was used for serum extraction for biochemical assays.

Results: By day seven post infection, all the birds in the infected group showed clinical signs associated with fowl cholera such as ruffled feathers, sneezing, respiratory distress, greenish-yellowish diarrhea, weakness, nasal and ocular discharges.

Conclusion: It was concluded that Significant (p ≤ 0.05)increase in mean serum concentrations of interferon Gama (IFNγ) and interleukine 1(IL-1β) with significant heterophilia, lymphocytosis and monocytosis signified possible antigenic stimulation of the immune system in response to the experimental infection with Pasteurella multocida serotype A:1.

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